Raw and processed data for Publication Sensor-integrated gut-on-a-chip for Monitoring senescence-mediated changes in the intestinal barrier
Description
Raw and processed data for Publication Sensor-integrated gut-on-a-chip for Monitoring senescence-mediated changes in the intestinal barrier, DOI: 10.1039/d4lc00896k
The Folders correspond to the raw and processed data visualized in the figures of the main article and the supporting Information.
Structure
The Folder structure is organized based on the figures in the publication and contains:
- Impedance measurments and processed data stored as .txt, .xlsx and .prism files
- Plate Reader Export files as .csv
- FITC Diffusion data processed and stored as .xlsx and .pzfx (GraphPad) files.
- Flourescence Imaging data stored as .tiff files
- Cell size distributions as .jpg and .xlsx files
- RT-qPCR raw files (melting curves, Ct values, run parameters) and processing data as .xls and .prism files
In the subfolders where additional explanation of the dataset is required .txt files are added
Remarks
The PDF documents are additionally included in the PDF/A format.
The PRISM and PZFX files can be viewed with the GraphPad Prism software.
The Python script was tested with Python 3.12, the dependencies are listed in requirements.txt.
Licenses
The data is licensed under CC-BY, the code is licensed under MIT.
Abstract (English)
The incidence of inflammatory bowel disease among the elderly has significantly risen in recent years, posing a growing socioeconomic burden to aging societies.
Moreover, non-gastrointestinal diseases, also prevalent in this demographic, have been linked to intestinal barrier dysfunction, thus highlighting the importance of investigating aged-mediated changes within the human gut.
While gastrointestinal pathology often involves an impaired gut barrier, the impact of aging on the human gastrointestinal barrier function remains unclear.
To explore the effect of senescence, a key hallmark of aging, on gut barrier integrity, we established and evaluated an in vitro gut-on-a-chip model tailored to investigate barrier changes by the integration of an impedance sensor.
Here, a microfluidic gut-on-a-chip system containing integrated membrane-based electrode microarrays is used to non-invasively monitor epithelial barrier formation and senescence-mediated changes in barrier integrity upon treating Caco-2 cells with 0.8 μg mL^−1 doxorubicin (DXR), a chemotherapeutic which induces cell cycle arrest.
Results of our microfluidic human gut model reveal a DXR-mediated increase in impedance and cell hypertrophy as well as overexpression of p21, and CCL2, indicative of a senescent phenotype.
Combined with the integrated electrodes, monitoring ∼57% of the cultivation area in situ and non-invasively, the developed chip-based senescent-gut model is ideally suited to study age-related malfunctions in barrier integrity.
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Additional details
Additional titles
- Alternative title
- Raw and processed data for DOI: 10.1039/d4lc00896k
Related works
- Is part of
- Other: https://endotargetproject.eu/ (URL)
- Is referenced by
- Journal Article: 10.1039/d4lc00896k (DOI)