Dataset for Publication Using coffee waste as feedstock for continuous cultivation of Sulfolobus acidocaldarius

Description of Datasets

1. "Table1_Supplementary Figure 1_Raw"
   
   • Raw data of sugar and inhibitor concentrations during the different hydrolysis conditions given in g/L
   • 3 Centerpoints of DoE setup are highlighted in yellow
   • Energy consumption is given in kwh for selected hydrolysates 
2. "Table2__Supplementary Figure2_Supplementary Table2_Raw"
   • Raw data of cultivation on chemical defined sugars in combination with monosodium glutamate and a mixture of the sugars and monosodium glutamate
   • Nomenclature: I = Replicate 1; II = Replicate 2; Glc = Sugar source: Glucose; Gal = Sugar source: Galactose; Man = Sugar source: Mannose; Ara = Sugar source: Arabinose; Mix = Sugar source: Mixture of Glc+Gal+Man+Ara;
   • OD600 data over time are shown
   • Sugar and Glutamate concentrations over time are shown (important for Supplementary Figure 2 and Supplementary Table 2)
   • Calculations as µmax and qSmax are shown for each flasks are shown
3. "Figure1_Raw"
   • OD600 data over time of Control (no treatment) vs. treatment with 3 different concentrations of AA, FF, and 5-HMF respectively 
4. "Table3_Supplementary Figure 5_Raw"
   • Raw data of continuous fermentation using chemically defined sugar feeds (glucose, galactose and mixture of glucose, galactose, mannose, and arabinose as Feed medium)
   • Structure: 
     • "Raw Data" contains all reactor raw data obtained from tower ("F12 Labfors 5"), offgas sensor ("BlueInOne"), and scales ("WIRC xy") over process-time
     • "OD600DCW" contains OD600 and DCW measurements over the entire process
     • "Metabolites" contains all metabolites traced during fermentation 
5. "Figure2_Table4_Raw"
   
   • Raw data of continuous fermentation using first chemically defined sugar feed (MSG+glucose) and then first SCG feed unmodified and later SCG feed with reduced salt content
   • Structure: 
     • "Raw Data" contains all reactor raw data obtained from tower ("F12 Labfors 5"), offgas sensor ("BlueInOne"), and scales ("WIRC xy") over process-time
     • "OD600DCW" contains OD600 and DCW measurements over the entire process
     • "Metabolites" contains all metabolites traced during fermentation 
6. "Supplementary Figure1_Raw"
   • Contains OD600 data over time comparing standard VD Medium (control) with 4 selected hydrolysates (VD+T121t1m10; VDT60t1m20; VD+T80t3.5m10; VD+T100t6m20)
7. "Supplementary Figure3_Raw"
   • Contains concentrations of AA, 5-HMF, and FF over time when incubated at process conditions without cells over time
8. "Supplementary Figure4_Raw"
   • Contains raw data of chromatogramms of flasks containing cells and either AA, 5-HMF, or FF showing degration of those substances over time when cells are present 
9. "Supplementary Figure7_Raw"
   • Contains raw data of cyclopentane ring distribution comparing standard fermentation grown samples on VD medium vs. SCG grown cells